Genes may inhibit replication and transcription of virus
Phoenix, AZ, and Washington, DC April 25, 2005--Using advanced
genetic technology, scientists have identified a set of genes that
become activated in individuals with HIV-1. These genes may be
potential drug targets that one day may lead to therapies that
block the progression of the disease. The findings were published
recently in the online issue of Retrovirology.
HIV infection and the subsequent development of AIDS are caused by
a number of factors including the "hijacking" of cellular
functions, which allows the infection to evade immune response.
After the body becomes immunodeficient, one protein in particular,
HIV-1 Tat, appears to be among the most critical for viral
transcription and replication.
Building upon this knowledge, scientists at the Translational
Genomics Research Institute (TGen), The George Washington
University School of Medicine, Johns Hopkins Medical School,
Tampere University of Technology, and the Institute for Genomic
Research used expression profiling technology to identify genes
that are activated by HIV-1 infection.
"The genes activated by HIV-1 are never turned on in healthy
adults. Therefore, these genes are ideal targets for therapy
because they are not crucial for normal human functions," said the
paper's senior author Fatah Kashanchi, an Associate Professor at
the George Washington University School of Medicine.
Using siRNA technology, researchers shut off the "activated" genes
and inhibited the ability of HIV-1 to replicate. While some of
these genes have already been established as critical for HIV-1
infection and replication, others have not and thus may serve as
novel therapeutic targets to be further studied.
"This study is an ongoing attempt to figure out which genes are
critical to Tat regulation. This allows us to define a set of
potential drug targets that will supplement current HIV therapies,"
said Dr. Dietrich Stephan, head of TGen's Neurogenomics
Division.
The earliest known case of HIV-1 in a human was from a blood sample
collected in 1959 from a man in Kinshasa, Democratic Republic of
Congo. Genetic analysis of this blood sample suggested that HIV-1
may have stemmed from a single virus in the late 1940s or early
1950s. The Centers for Disease Control and Prevention estimate that
850,000 to 950,000 U.S. residents are living with HIV infection,
one-quarter of who are unaware of their infection. Approximately
40,000 new HIV infections occur each year in the United States,
about 70 percent among men and 30 percent among women. Of these
newly infected people, half are younger than 25 years of age
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About TGen
The mission of the Translational Genomics Research Institute (TGen)
is to make and translate genomic discoveries into advances in human
health. Translational genomics research is a relatively new field
employing innovative advances arising from the Human Genome Project
and applying them to the development of diagnostics, prognostics
and therapies for cancer, neurological disorders, diabetes and
other complex diseases. TGen is focused on personalized medicine
and plans to accomplish its goals through robust and
disease-focused research programs and its state-of-the-art
bioinformatics and computational biology facilities.
About The George Washington University
Located four blocks from the White House, The George Washington
University was created by an Act of Congress in 1821. Today, GW is
the largest institution of higher education in the nation's
capital. The University offers comprehensive programs of
undergraduate and graduate liberal arts study as well as degree
programs in medicine, law, engineering, education, business, and
international affairs. Each year, GW enrolls a diverse population
of undergraduate, graduate, and professional students from all 50
states, the District of Columbia, and more than 120
countries.
Media Contacts:
Galen Perry (602) 343-8423
Amy Erickson (602) 343-8522