Miniaturized paper-based vertical flow immunoassay (miniVFI) for diagnosis of melioidosis
Melioidosis, an infectious disease caused by the bacterium Burkholderia pseudomallei, has a 40% mortality rate highly affecting areas in Southeast Asia and Northern Australia. Traditional diagnostics such as microbiological culturing and PCR have limitations such as time constraints, high costs, low sensitivity, and the need for trained personnel. Therefore, there is a need for the development of new diagnostics tools in order to overcome these limitations at point-of-care settings. We developed a miniaturized paper-based vertical flow immunoassay system in which a 13 mm nitrocellulose membrane was reduced to a 3.5 mm membrane in order to increase sensitivity. By reducing the surface area of the membrane, the sample volume per unit area was increased achieving an increase of 2.3 times in signal intensity.